Journal: Cells

Article Title: Human Extravillous Trophoblasts Require SRC-2 for Sustained Viability, Migration, and Invasion

doi: 10.3390/cells14131024

Figure Lengend Snippet: Dysregulated expression of WNT signaling factors in HTR-8/SVneo cells following SRC-2 knockdown: ( A ) Heat map displays marked changes in the expression of genes associated with WNT signaling between the NT siRNA and SRC-2 siRNA replicate groups. While WNT signaling inhibitors, Dickkopf WNT signaling pathway inhibitor 1 ( DKK1 ) and secreted frizzled related protein 1 ( SFRP1 ), are upregulated in the SRC-2 siRNA-treated group, the expression levels of Wnt family member 2 ( WNT2 ), WNT4 , WNT6 , WNT8B , and WNT9A are significantly reduced. ( B ) Confirmation by qRT-PCR of the above-described gene expression changes between the NT siRNA and SRC-2 siRNA-treated groups is shown. An explanation of the average fold change (FC ± standard deviation) calculation is described in the . Results are representative of three independent experiments; * p -value < 0.05; ** p -value < 0.01, and *** p value < 0.001.

Article Snippet: WNT2 , 7472 , Hs00608224_m1.

Techniques: Expressing, Knockdown, Quantitative RT-PCR, Gene Expression, Standard Deviation

Journal: Pharmacology Research & Perspectives

Article Title: Lobaric Acid Exhibits Anticancer Potential by Modulating the Wnt/β‐Catenin Signaling Pathway in MCF‐7 Cells

doi: 10.1002/prp2.70142

Figure Lengend Snippet: Effect of LA on Wnt/β‐catenin pathway in MCF‐7 cells. (A) The migration of cells treated with LA into the wound was visualized at 0, 6, 12, and 24 h under an inverted microscope, and the percentage of wound closure was evaluated statistically. (B–I) Expressions of WNT2 , DVL1 , AXIN1 , β‐Catenin , TCF‐4 , CCND1 , c‐MYC , and CDK1 genes analyzed by qPCR in LA‐treated MCF‐7 cells at 48 h. (J–M) Protein expressions of WNT2, GSK3‐ β, and β‐catenin determined by Western blot analysis. The experiment was performed in three biological and technical replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001 in relation to the control. Scale bar, 500 μm.

Article Snippet: Subsequently, membrane incubation was performed overnight at 4°C using P53 antibody (Proteintech, 10442‐1‐AP, 1:1000), BCL2 antibody (Proteintech 12789‐1‐AP 1:500), WNT2 antibody (Proteintech, 66656‐I‐Ig, 1:1000), AXIN antibody (Proteintech, 68093‐1‐Ig, 1:1000), β‐Catenin antibody (Santa Cruz Biotechnology, sc‐7963, 1:1000), GSK3‐ β antibody (Santa Cruz, sc‐377213, 1:1000), and β‐Actin antibody (Santa Cruz Biotechnology, sc‐47778, 1:1000).

Techniques: Migration, Inverted Microscopy, Western Blot, Control